The Stagonospora Nodorum Groupings Identified in The Field Exhibit Variations in Cultural Characteristics, Pathogenicity, Vegetative Compatibility, and Electrophoretic Karyotype.

Abstract

The infectious agent that causes septoria leaf and glume blotch, Stagonospora nodorum, was the subject of an extensive two-year investigation. The investigation aimed to evaluate a large number of fungal isolates. Electrophoretic karyotype, compatibility in vegetative reproduction, virulence, colon shape, and rate of development were the main areas of comparison. A thorough inspection of multiple wheat fields was part of our investigation. Significant heterogeneity was identified in all characteristics between the two groups, even though the isolates in each population came from a very small geographical area. Vegetative compatibility study within each group also revealed a wide variety of genotypes and showed that clone expansion was severely limited. The fact that the genotypes of the lesions were different strongly suggests that they were all caused by the same inoculum. Reason being, different genotypes were found in each lesion. On the other hand, a broad range of genotypes was frequently observed in lesions that were either on the same leaf or quite adjacent to each other. This was the norm even though they were all on the same planet. Researchers found six different karyotypes after examining 10 isolates from population II. This discovery shows that there is a lot of genetic variation in field populations when it comes to the crucial trait. According to the results of this study on population structure, ascospores are crucial to the disease's epidemiology. So, karyotype diversity, along with all the other traits, may be explained by the recombination process that happens during sexual reproduction. This is due to the fact that sexual reproduction cannot occur without the merging of sperm and egg.

Keywords: September nodorum, glume blotch on wheat, field groups, vegetative incompatibility, sexual reproduction